MY Polymers

Direct characterization of the evanescent field in objective-type total internal reflection fluorescence microscopy

(Christian Niederauer et al, August 2018)

Description:

This publication describes the use of MY Polymers MY-133-MC (index=1.33) for producing a calibration slide that enables to characterize the excitation field in Total Internal Reflection Fluorescence Microscopy. The slide is coated by gradually thicker layers of MY-133-MC. This is done by dip coating the slide several times, by partially overlapping layers of the material.

(MY-133-MC is Moisture Cured. There is no need for a UV light source and inert atmosphere).

 

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A polymer gel index-matched to water enables diverse applications in fluorescence imaging

(Hari Shroff et al, October 2020)

Description:

This publication shows how BIO-133 can be used to enable multiple applications in fluorescence imaging.

The research covers the following issues:

(1) Characterization of BIO-133 for optical imaging in microfluidic/immobilization setups;

(2) Showing that diffraction-limited and super-resolution imaging is possible when using this material;

(3) Showing that high-resolution light-sheet microscopy with water-dipping objectives is fully compatible with microfluidic setups, a goal thought by many in the field to be impossible;

 

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