Direct characterization of the evanescent field in objective-type total internal reflection fluorescence microscopy
(Christian Niederauer et al, August 2018)
Description:
This publication describes the use of MY Polymers MY-133-MC (index=1.33) for producing a calibration slide that enables to characterize the excitation field in Total Internal Reflection Fluorescence Microscopy. The slide is coated by gradually thicker layers of MY-133-MC. This is done by dip coating the slide several times, by partially overlapping layers of the material.
(MY-133-MC is Moisture Cured. There is no need for a UV light source and inert atmosphere).